Analysis Note

Positive ControlRat hypothalamus

Application

Frozen Sections (1:400-1:800, Cy3; 1:2000-1:4000, Bn-SA/HRP)Immunofluorescence (1:200-1:400)

General description

Recognizes vasopressin in rat hypothalamus.

Rabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes vasopressin.

This Anti-Vasopressin Rabbit pAb is validated for use in Frozen Sections, Immunofluorescence for the detection of Vasopressin.

Immunogen

rat arginine vasopressin

Rat

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Sudhof, T.C. 1995. Nature375, 645.Hokfelt, T., et al. 1987. Experientia Suppl.56, 154.

Antibody specificity was examined in the rat paraventricular nucleus and supraoptic nucleus. Staining is completely eliminated by pretreatment of antibody with arginine vasopressin (10 µg/ml). Preabsorption of antibody with oxytocin (100 µg/ml) resulted in no loss of immunostaining. Antibody should be titrated for optimal results in individual systems.

Packaging

100 µL in Plastic ampoule

Physical form

Undiluted serum.

Reconstitution

Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months. Avoid freeze/thaw cycles of solutions.

Warning

Toxicity: Standard Handling (A)